Shahrzad Abbasi, MS
Texas Heart Institute Positions
- Research Associate I, Molecular Cardiology Research
Interests
- Non-invasive diagnostic imaging technologies
Publications
4862227
JH9V95Q8
1
alternatives-to-animal-experimentation
10
date
desc
Abbasi
1795
https://www.texasheart.org/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22U98WXEYF%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ma%20et%20al.%22%2C%22parsedDate%22%3A%222020-08-21%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMa%2C%20Y.%2C%20Cheng%2C%20N.%2C%20Sun%2C%20J.%20et%20al.%20%282020%29.%20Atherogenic%20L5%20LDL%20induces%20cardiomyocyte%20apoptosis%20and%20inhibits%20KATP%20channels%20through%20CaMKII%20activation.%20%3Ci%3ELipids%20Health%20Dis%3C%5C%2Fi%3E%20%3Ci%3E19%3C%5C%2Fi%3E%2C%20189.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12944-020-01368-7%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12944-020-01368-7%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Atherogenic%20L5%20LDL%20induces%20cardiomyocyte%20apoptosis%20and%20inhibits%20KATP%20channels%20through%20CaMKII%20activation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yanzhuo%22%2C%22lastName%22%3A%22Ma%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nancy%22%2C%22lastName%22%3A%22Cheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Junping%22%2C%22lastName%22%3A%22Sun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%20Xuhai%22%2C%22lastName%22%3A%22Lu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Geru%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22An-Sheng%22%2C%22lastName%22%3A%22Lee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tatsuya%22%2C%22lastName%22%3A%22Sawamura%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jie%22%2C%22lastName%22%3A%22Cheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chu-Huang%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yutao%22%2C%22lastName%22%3A%22Xi%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Cardiac%20Ca2%2B%5C%2Fcalmodulin-dependent%20protein%20kinase%20II%20%28CaMKII%29%20activation%20plays%20a%20critical%20role%20in%20cardiomyocyte%20%28CM%29%20apoptosis%20and%20arrhythmia.%20Functional%20ATP-sensitive%20potassium%20%28KATP%29%20channels%20are%20essential%20for%20cardiac%20protection%20during%20ischemia.%20In%20cultured%20CMs%2C%20L5%20low-density%20lipoprotein%20%28LDL%29%20induces%20apoptosis%20and%20QTc%20prolongation.%20L5%20is%20a%20highly%20electronegative%20and%20atherogenic%20aberrant%20form%20of%20LDL%2C%20and%20its%20levels%20are%20significantly%20higher%20in%20patients%20with%20cardiovascular-related%20diseases.%20Here%2C%20the%20role%20of%20L5%20in%20cardiac%20injury%20was%20studied%20by%20evaluating%20the%20effects%20of%20L5%20on%20CaMKII%20activity%20and%20KATP%20channel%20physiology%20in%20CMs.%5CnMETHODS%3A%20Cultured%20neonatal%20rat%20CMs%20%28NRCMs%29%20were%20treated%20with%20a%20moderate%20concentration%20%28ie%2C%207.5%5Cu2009%5Cu03bcg%5C%2FmL%29%20of%20L5%20or%20L1%20%28the%20least%20electronegative%20LDL%20subfraction%29.%20NRCMs%20were%20examined%20for%20apoptosis%20and%20viability%2C%20CaMKII%20activity%2C%20and%20the%20expression%20of%20phosphorylated%20CaMKII%5Cu03b4%20and%20NOX2%5C%2Fgp91phox.%20The%20function%20of%20KATP%20and%20action%20potentials%20%28APs%29%20was%20analyzed%20by%20using%20the%20patch-clamp%20technique.%5CnRESULTS%3A%20In%20NRCMs%2C%20L5%20but%20not%20L1%20significantly%20induced%20cell%20apoptosis%20and%20reduced%20cell%20viability.%20Furthermore%2C%20L5%20decreased%20Kir6.2%20expression%20by%20more%20than%2050%25.%20Patch-clamp%20analysis%20showed%20that%20L5%20reduced%20the%20KATP%20current%20%28IKATP%29%20density%20induced%20by%20pinacidil%2C%20a%20KATP%20opener.%20The%20partial%20recovery%20of%20the%20inward%20potassium%20current%20during%20pinacidil%20washout%20was%20susceptible%20to%20subsequent%20inhibition%20by%20the%20IKATP%20blocker%20glibenclamide.%20Suppression%20of%20IKATP%20by%20L5%20significantly%20prolonged%20the%20AP%20duration.%20L5%20also%20significantly%20increased%20the%20activity%20of%20CaMKII%2C%20the%20phosphorylation%20of%20CaMKII%5Cu03b4%2C%20and%20the%20expression%20of%20NOX2%5C%2Fgp91phox.%20L5-induced%20apoptosis%20was%20prevented%20by%20the%20addition%20of%20the%20CaMKII%20inhibitor%20KN93%20and%20the%20reactive%20oxygen%20species%20scavenger%20Mn%20%28III%29TBAP.%5CnCONCLUSIONS%3A%20L5%20but%20not%20L1%20induces%20CM%20damage%20through%20the%20activation%20of%20the%20CaMKII%20pathway%20and%20increases%20arrhythmogenicity%20in%20CMs%20by%20modulating%20the%20AP%20duration.%20These%20results%20help%20to%20explain%20the%20harmful%20effects%20of%20L5%20in%20cardiovascular-related%20disease.%22%2C%22date%22%3A%22Aug%2021%2C%202020%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1186%5C%2Fs12944-020-01368-7%22%2C%22ISSN%22%3A%221476-511X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22IVWSNHMB%22%2C%228EPJLFD3%22%2C%22JH9V95Q8%22%2C%22RB23UBKZ%22%5D%2C%22dateModified%22%3A%222020-09-15T17%3A41%3A07Z%22%7D%7D%2C%7B%22key%22%3A%229LRIQ9XJ%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Woodside%20et%20al.%22%2C%22parsedDate%22%3A%222018-02-27%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWoodside%2C%20D.%20G.%2C%20Tanifum%2C%20E.%20A.%2C%20Ghaghada%2C%20K.%20B.%20et%20al.%20%282018%29.%20Magnetic%20resonance%20imaging%20of%20atherosclerotic%20plaque%20at%20clinically%20relevant%20field%20strengths%20%281T%29%20by%20targeting%20the%20integrin%20%26%23x3B1%3B4%26%23x3B2%3B1.%20%3Ci%3ESci%20Rep%3C%5C%2Fi%3E%20%3Ci%3E8%3C%5C%2Fi%3E%2C%203733.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-018-21893-x%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-018-21893-x%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Magnetic%20resonance%20imaging%20of%20atherosclerotic%20plaque%20at%20clinically%20relevant%20field%20strengths%20%281T%29%20by%20targeting%20the%20integrin%20%5Cu03b14%5Cu03b21%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Darren%20G.%22%2C%22lastName%22%3A%22Woodside%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20A.%22%2C%22lastName%22%3A%22Tanifum%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ketan%20B.%22%2C%22lastName%22%3A%22Ghaghada%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ronald%20J.%22%2C%22lastName%22%3A%22Biediger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amy%20R.%22%2C%22lastName%22%3A%22Caivano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zbigniew%20A.%22%2C%22lastName%22%3A%22Starosolski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sayadeth%22%2C%22lastName%22%3A%22Khounlo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Saakshi%22%2C%22lastName%22%3A%22Bhayana%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20W.%22%2C%22lastName%22%3A%22Craft%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20S.%22%2C%22lastName%22%3A%22Maxwell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chandreshkumar%22%2C%22lastName%22%3A%22Patel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Igor%20V.%22%2C%22lastName%22%3A%22Stupin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Deenadayalan%22%2C%22lastName%22%3A%22Bakthavatsalam%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20V.%22%2C%22lastName%22%3A%22Market%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22James%20T.%22%2C%22lastName%22%3A%22Willerson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Richard%20A.%20F.%22%2C%22lastName%22%3A%22Dixon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Vanderslice%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ananth%20V.%22%2C%22lastName%22%3A%22Annapragada%22%7D%5D%2C%22abstractNote%22%3A%22Inflammation%20drives%20the%20degradation%20of%20atherosclerotic%20plaque%2C%20yet%20there%20are%20no%20non-invasive%20techniques%20available%20for%20imaging%20overall%20inflammation%20in%20atherosclerotic%20plaques%2C%20especially%20in%20the%20coronary%20arteries.%20To%20address%20this%2C%20we%20have%20developed%20a%20clinically%20relevant%20system%20to%20image%20overall%20inflammatory%20cell%20burden%20in%20plaque.%20Here%2C%20we%20describe%20a%20targeted%20contrast%20agent%20%28THI0567-targeted%20liposomal-Gd%29%20that%20is%20suitable%20for%20magnetic%20resonance%20%28MR%29%20imaging%20and%20binds%20with%20high%20affinity%20and%20selectivity%20to%20the%20integrin%20%5Cu03b14%5Cu03b21%28very%20late%20antigen-4%2C%20VLA-4%29%2C%20a%20key%20integrin%20involved%20in%20recruiting%20inflammatory%20cells%20to%20atherosclerotic%20plaques.%20This%20liposomal%20contrast%20agent%20has%20a%20high%20T1%20relaxivity%20%28~2%5Cu2009%5Cu00d7%5Cu2009105%5Cu2009mM-1s-1%20on%20a%20particle%20basis%29%20resulting%20in%20the%20ability%20to%20image%20liposomes%20at%20a%20clinically%20relevant%20MR%20field%20strength.%20We%20were%20able%20to%20visualize%20atherosclerotic%20plaques%20in%20various%20regions%20of%20the%20aorta%20in%20atherosclerosis-prone%20ApoE-%5C%2F-%20mice%20on%20a%201%20Tesla%20small%20animal%20MRI%20scanner.%20These%20enhanced%20signals%20corresponded%20to%20the%20accumulation%20of%20monocyte%5C%2Fmacrophages%20in%20the%20subendothelial%20layer%20of%20atherosclerotic%20plaques%20in%20vivo%2C%20whereas%20non-targeted%20liposomal%20nanoparticles%20did%20not%20demonstrate%20comparable%20signal%20enhancement.%20An%20inflammatory%20cell-targeted%20method%20that%20has%20the%20specificity%20and%20sensitivity%20to%20measure%20the%20inflammatory%20burden%20of%20a%20plaque%20could%20be%20used%20to%20noninvasively%20identify%20patients%20at%20risk%20of%20an%20acute%20ischemic%20event.%22%2C%22date%22%3A%22Feb%2027%2C%202018%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-018-21893-x%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22NNUFWYNJ%22%2C%226MADD4J5%22%2C%22WGZKEVDQ%22%2C%22BBMMGS3D%22%2C%227XFQVVGN%22%2C%22JH9V95Q8%22%2C%22VXDM6BAE%22%2C%224R4R39JD%22%2C%227STYFUFZ%22%2C%225QIRWFUY%22%5D%2C%22dateModified%22%3A%222018-05-07T19%3A32%3A11Z%22%7D%7D%2C%7B%22key%22%3A%22F65WUXH8%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Xi%20et%20al.%22%2C%22parsedDate%22%3A%222015-06%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EXi%2C%20Y.%2C%20James%20Chao%2C%20Z.-Y.%2C%20Yan%2C%20W.%20et%20al.%20%282015%29.%20Neuronally%20released%20vasoactive%20intestinal%20polypeptide%20alters%20atrial%20electrophysiological%20properties%20and%20may%20promote%20atrial%20fibrillation.%20%3Ci%3EHeart%20Rhythm%3C%5C%2Fi%3E%20%3Ci%3E12%3C%5C%2Fi%3E%2C%201352%26%23x2013%3B1361.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.hrthm.2015.03.003%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.hrthm.2015.03.003%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Neuronally%20released%20vasoactive%20intestinal%20polypeptide%20alters%20atrial%20electrophysiological%20properties%20and%20may%20promote%20atrial%20fibrillation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yutao%22%2C%22lastName%22%3A%22Xi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhi-Yang%22%2C%22lastName%22%3A%22James%20Chao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wen%22%2C%22lastName%22%3A%22Yan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaomeng%22%2C%22lastName%22%3A%22Yin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nilesh%22%2C%22lastName%22%3A%22Mathuria%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mehul%22%2C%22lastName%22%3A%22Patel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Fan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Junping%22%2C%22lastName%22%3A%22Sun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Geru%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Suwei%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22MacArthur%22%2C%22lastName%22%3A%22Elayda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lianjun%22%2C%22lastName%22%3A%22Gao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xander%20H.%20T.%22%2C%22lastName%22%3A%22Wehrens%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shien-Fong%22%2C%22lastName%22%3A%22Lin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jie%22%2C%22lastName%22%3A%22Cheng%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Vagal%20hyperactivity%20promotes%20atrial%20fibrillation%20%28AF%29%2C%20which%20has%20been%20almost%20exclusively%20attributed%20to%20acetylcholine.%20Vasoactive%20intestinal%20polypeptide%20%28VIP%29%20and%20acetylcholine%20are%20neurotransmitters%20co-released%20during%20vagal%20stimulation.%20Exogenous%20VIP%20has%20been%20shown%20to%20promote%20AF%20by%20shortening%20action%20potential%20duration%20%28APD%29%2C%20increasing%20APD%20spatial%20heterogeneity%2C%20and%20causing%20intra-atrial%20conduction%20block.%5CnOBJECTIVE%3A%20The%20purpose%20of%20this%20study%20was%20to%20investigate%20the%20effects%20of%20neuronally%20released%20VIP%20on%20atrial%20electrophysiologic%20properties%20during%20vagal%20stimulation.%5CnMETHODS%3A%20We%20used%20a%20specific%20VIP%20antagonist%20%28H9935%29%20to%20uncover%20the%20effects%20of%20endogenous%20VIP%20released%20during%20vagal%20stimulation%20in%20canine%20hearts.%5CnRESULTS%3A%20H9935%20significantly%20attenuated%20%281%29%20the%20vagally%20induced%20shortening%20of%20atrial%20effective%20refractory%20period%20and%20widening%20of%20atrial%20vulnerability%20window%20during%20stimulation%20of%20cervical%20vagosympathetic%20trunks%20%28VCNS%29%20and%20%282%29%20vagal%20effects%20on%20APD%20during%20stimulation%20through%20fat-pad%20ganglion%20plexus%20%28VGPS%29.%20Atropine%20completely%20abolished%20these%20vagal%20effects%20during%20VCNS%20and%20VGPS.%20In%20contrast%2C%20VGPS-induced%20slowing%20of%20local%20conduction%20velocity%20was%20completely%20abolished%20by%20either%20VIP%20antagonist%20or%20atropine.%20In%20pacing-induced%20AF%20during%20VGPS%2C%20maximal%20dominant%20frequencies%20and%20their%20spatial%20gradients%20were%20reduced%20significantly%20by%20H9935%20and%2C%20more%20pronouncedly%2C%20by%20atropine.%20Furthermore%2C%20VIP%20release%20in%20the%20atria%20during%20vagal%20stimulation%20was%20inhibited%20by%20atropine%2C%20which%20may%20account%20for%20the%20concealment%20of%20VIP%20effects%20with%20muscarinic%20blockade.%5CnCONCLUSION%3A%20Neuronally%20released%20VIP%20contributes%20to%20vagal%20effects%20on%20atrial%20electrophysiologic%20properties%20and%20affects%20the%20pathophysiology%20of%20vagally%20induced%20AF.%20Neuronal%20release%20of%20VIP%20in%20the%20atria%20is%20inhibited%20by%20muscarinic%20blockade%2C%20a%20novel%20mechanism%20by%20which%20VIP%20effects%20are%20concealed%20by%20atropine%20during%20vagal%20stimulation.%22%2C%22date%22%3A%22Jun%202015%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.hrthm.2015.03.003%22%2C%22ISSN%22%3A%221556-3871%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%228EPJLFD3%22%2C%22CEPAWXUI%22%2C%22JH9V95Q8%22%2C%22RB23UBKZ%22%2C%224VC6SC4L%22%5D%2C%22dateModified%22%3A%222022-02-08T19%3A57%3A17Z%22%7D%7D%2C%7B%22key%22%3A%228VQQLJJ3%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sun%20et%20al.%22%2C%22parsedDate%22%3A%222014-09-20%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESun%2C%20J.%2C%20Zhang%2C%20J.%2C%20Yan%2C%20W.%20et%20al.%20%282014%29.%20Iloprost%20prevents%20doxorubicin%20mediated%20human%20cardiac%20progenitor%20cell%20depletion.%20%3Ci%3EInt%20J%20Cardiol%3C%5C%2Fi%3E%20%3Ci%3E176%3C%5C%2Fi%3E%2C%20536%26%23x2013%3B539.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ijcard.2014.07.031%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ijcard.2014.07.031%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iloprost%20prevents%20doxorubicin%20mediated%20human%20cardiac%20progenitor%20cell%20depletion%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Junping%22%2C%22lastName%22%3A%22Sun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jia%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wen%22%2C%22lastName%22%3A%22Yan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cai%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Geru%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bao%22%2C%22lastName%22%3A%22Pham%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Steven%22%2C%22lastName%22%3A%22Lee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jie%22%2C%22lastName%22%3A%22Cheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nada%20B.%22%2C%22lastName%22%3A%22Memon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yutao%22%2C%22lastName%22%3A%22Xi%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%22Sep%2020%2C%202014%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.ijcard.2014.07.031%22%2C%22ISSN%22%3A%221874-1754%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%228EPJLFD3%22%2C%22JH9V95Q8%22%2C%22RB23UBKZ%22%2C%224VC6SC4L%22%5D%2C%22dateModified%22%3A%222018-04-26T19%3A46%3A13Z%22%7D%7D%2C%7B%22key%22%3A%226A8IEPGF%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Xi%20et%20al.%22%2C%22parsedDate%22%3A%222013-10%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EXi%2C%20Y.%2C%20Wu%2C%20G.%2C%20Ai%2C%20T.%20et%20al.%20%282013%29.%20Ionic%20mechanisms%20underlying%20the%20effects%20of%20vasoactive%20intestinal%20polypeptide%20on%20canine%20atrial%20myocardium.%20%3Ci%3ECirc%20Arrhythm%20Electrophysiol%3C%5C%2Fi%3E%20%3Ci%3E6%3C%5C%2Fi%3E%2C%20976%26%23x2013%3B983.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2FCIRCEP.113.000518%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2FCIRCEP.113.000518%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Ionic%20mechanisms%20underlying%20the%20effects%20of%20vasoactive%20intestinal%20polypeptide%20on%20canine%20atrial%20myocardium%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yutao%22%2C%22lastName%22%3A%22Xi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Geru%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tomohiko%22%2C%22lastName%22%3A%22Ai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nancy%22%2C%22lastName%22%3A%22Cheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jurij%20Matija%22%2C%22lastName%22%3A%22Kalisnik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Junping%22%2C%22lastName%22%3A%22Sun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Donghui%22%2C%22lastName%22%3A%22Yang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Fan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaojing%22%2C%22lastName%22%3A%22Yuan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Suwei%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22MacArthur%22%2C%22lastName%22%3A%22Elayda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Igor%20D.%22%2C%22lastName%22%3A%22Gregoric%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bharat%20K.%22%2C%22lastName%22%3A%22Kantharia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shien-Fong%22%2C%22lastName%22%3A%22Lin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jie%22%2C%22lastName%22%3A%22Cheng%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Vasoactive%20intestinal%20polypeptide%20%28VIP%29%20is%20released%20from%20intracardiac%20neurons%20during%20vagal%20stimulation%2C%20ischemia%2C%20and%20heart%20failure%2C%20which%20are%20associated%20with%20increased%20vulnerability%20to%20atrial%20fibrillation.%20VIP%20shortens%20atrial%20effective%20refractory%20periods%20in%20dogs.%20Endogenous%20VIP%20contributes%20to%20vagally%20mediated%20acceleration%20of%20atrial%20electric%20remodeling.%20VIP%20is%20also%20shown%20to%20prolong%20the%20duration%20of%20acetylcholine-induced%20atrial%20fibrillation.%20However%2C%20the%20ionic%20mechanisms%20underlying%20VIP%20effects%20are%20largely%20unknown.%5CnMETHODS%20AND%20RESULTS%3A%20The%20effects%20of%20VIP%20on%20transmembrane%20ion%20channels%20were%20studied%20in%20canine%20atrial%20cardiomyocytes%20using%20patch-clamp%20techniques.%20VIP%20increased%20delayed%20rectifier%20K%2B%20current%20and%20L-type%20calcium%20current%20but%20decreased%20the%20transient%20outward%20K%2B%20current%20and%20sodium%20current.%20Optical%20mapping%20technique%20was%20used%20to%20assess%20effects%20of%20VIP%20on%20action%20potential%20durations%20%28APDs%29%20in%20isolated%20canine%20left%20atria.%20VIP%20shortened%20APD%20and%20slowed%20conduction%20velocity%20in%20a%20dose-dependent%20manner.%20Furthermore%2C%20VIP%20increased%20spatial%20heterogeneity%20of%20APD%20and%20conduction%20velocity%2C%20as%20assessed%20by%20the%20SDs%20of%20APD%20and%20conduction%20velocity%2C%20and%20atrial%20fibrillation%20inducibility.%5CnCONCLUSIONS%3A%20Through%20its%20diverse%20effects%20on%20ion%20channels%2C%20VIP%20shortens%20APD%20with%20increased%20APD%20spatial%20heterogeneity%20and%20decreases%20intra-atrial%20conduction%20velocity%2C%20which%20may%20play%20an%20important%20role%20in%20the%20pathogenesis%20of%20atrial%20arrhythmias%20in%20scenarios%20where%20VIP%20release%20is%20increased.%22%2C%22date%22%3A%22Oct%202013%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1161%5C%2FCIRCEP.113.000518%22%2C%22ISSN%22%3A%221941-3084%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%228EPJLFD3%22%2C%22JH9V95Q8%22%2C%22RB23UBKZ%22%2C%224VC6SC4L%22%5D%2C%22dateModified%22%3A%222018-04-26T19%3A46%3A13Z%22%7D%7D%2C%7B%22key%22%3A%22SD8H8KPX%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Barakat%20et%20al.%22%2C%22parsedDate%22%3A%222012-03%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBarakat%2C%20O.%2C%20%3Cstrong%3EAbbasi%3C%5C%2Fstrong%3E%2C%20S.%2C%20Rodriguez%2C%20G.%20et%20al.%20%282012%29.%20Use%20of%20decellularized%20porcine%20liver%20for%20engineering%20humanized%20liver%20organ.%20%3Ci%3EJ%20Surg%20Res%3C%5C%2Fi%3E%20%3Ci%3E173%3C%5C%2Fi%3E%2C%20e11-25.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jss.2011.09.033%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jss.2011.09.033%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Use%20of%20decellularized%20porcine%20liver%20for%20engineering%20humanized%20liver%20organ%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Omar%22%2C%22lastName%22%3A%22Barakat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gabriela%22%2C%22lastName%22%3A%22Rodriguez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessie%22%2C%22lastName%22%3A%22Rios%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20Patrick%22%2C%22lastName%22%3A%22Wood%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22Ozaki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laurie%20S.%22%2C%22lastName%22%3A%22Holley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Polly%20K.%22%2C%22lastName%22%3A%22Gauthier%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20New%20bioartificial%20liver%20devices%20are%20needed%20to%20supplement%20the%20limited%20supply%20of%20organ%20donors%20available%20for%20patients%20with%20end-stage%20liver%20disease.%20Here%2C%20we%20report%20the%20results%20of%20a%20pilot%20study%20aimed%20at%20developing%20a%20humanized%20porcine%20liver%20by%20transplanting%20second%20trimester%20human%20fetal%20hepatocytes%20%28Hfh%29%20co-cultured%20with%20fetal%20stellate%20cells%20%28Hfsc%29%20into%20the%20decellularized%20matrix%20of%20a%20porcine%20liver.%5CnMATERIAL%20AND%20METHODS%3A%20Ischemic%20livers%20were%20removed%20from%2019%20Yorkshire%20swine.%20Liver%20decellularization%20was%20achieved%20by%20an%20anionic%20detergent%20%28SDS%29.%20The%20decellularized%20matrix%20of%20three%20separate%20porcine%20liver%20matrices%20was%20seeded%20with%203.5%20%5Cu00d7%2010%288%29%20and%201%20%5Cu00d7%2010%289%29%20of%20Hfsc%20and%20Hfh%2C%20respectively%2C%20and%20perfused%20for%203%2C%207%2C%20and%2013%20d.%20The%20metabolic%20and%20synthetic%20activities%20of%20the%20engrafted%20cells%20were%20assessed%20during%20and%20after%20perfusion.%5CnRESULTS%3A%20Immunohistologic%20examination%20of%20the%20decellularized%20matrix%20showed%20removal%20of%20nuclear%20materials%20with%20intact%20architecture%20and%20preserved%20extracellular%20matrix%20%28ECM%29%20proteins.%20During%20perfusion%20of%20the%20recellularized%20matrices%2C%20measurement%20of%20metabolic%20parameters%20%28i.e.%2C%20oxygen%20concentration%2C%20glucose%20consumption%2C%20and%20lactate%20and%20urea%20production%29%20indicated%20active%20metabolism.%20The%20average%20human%20albumin%20concentration%20was%2029.48%20%5Cu00b1%207.4%20%5Cu03bcg%5C%2FmL.%20Immunohistochemical%20analysis%20revealed%20cell%20differentiation%20into%20mature%20hepatocytes.%20Moreover%2C%2040%25%20of%20the%20engrafted%20cells%20were%20actively%20proliferating%2C%20and%20less%20than%2030%25%20of%20cells%20were%20apoptotic.%5CnCONCLUSION%3A%20We%20showed%20that%20our%20decellularization%20protocol%20successfully%20removed%20the%20cellular%20components%20of%20porcine%20livers%20while%20preserving%20the%20native%20architecture%20and%20most%20ECM%20protein.%20We%20also%20demonstrated%20the%20ability%20of%20the%20decellularized%20matrix%20to%20support%20and%20induce%20phenotypic%20maturation%20of%20engrafted%20Hfh%20in%20a%20continuously%20perfused%20system.%22%2C%22date%22%3A%22Mar%202012%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jss.2011.09.033%22%2C%22ISSN%22%3A%221095-8673%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22ALPULI85%22%2C%22JH9V95Q8%22%2C%22XBMJSJKL%22%5D%2C%22dateModified%22%3A%222023-07-27T15%3A57%3A25Z%22%7D%7D%2C%7B%22key%22%3A%22V7GG4RNW%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zhou%20et%20al.%22%2C%22parsedDate%22%3A%222012%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZhou%2C%20C.%20C.%2C%20Chang%2C%20J.%2C%20Mi%2C%20T.%20et%20al.%20%282012%29.%20Targeted%20expression%20of%20Cre%20recombinase%20provokes%20placental-specific%20DNA%20recombination%20in%20transgenic%20mice.%20%3Ci%3EPLoS%20ONE%3C%5C%2Fi%3E%20%3Ci%3E7%3C%5C%2Fi%3E%2C%20e29236.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0029236%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0029236%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Targeted%20expression%20of%20Cre%20recombinase%20provokes%20placental-specific%20DNA%20recombination%20in%20transgenic%20mice%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cissy%20Chenyi%22%2C%22lastName%22%3A%22Zhou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiang%22%2C%22lastName%22%3A%22Chang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tiejuan%22%2C%22lastName%22%3A%22Mi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dongmin%22%2C%22lastName%22%3A%22Gu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Le%22%2C%22lastName%22%3A%22Huang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22WenZheng%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rodney%20E.%22%2C%22lastName%22%3A%22Kellems%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20J.%22%2C%22lastName%22%3A%22Schwartz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yang%22%2C%22lastName%22%3A%22Xia%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Inadequate%20placental%20development%20is%20associated%20with%20a%20high%20incidence%20of%20early%20embryonic%20lethality%20and%20serious%20pregnancy%20disorders%20in%20both%20humans%20and%20mice.%20However%2C%20the%20lack%20of%20well-defined%20trophoblast-specific%20gene%20regulatory%20elements%20has%20hampered%20investigations%20regarding%20the%20role%20of%20specific%20genes%20in%20placental%20development%20and%20fetal%20growth.%5CnPRINCIPAL%20FINDINGS%3A%20By%20random%20assembly%20of%20placental%20enhancers%20from%20two%20previously%20characterized%20genes%2C%20trophoblast%20specific%20protein%20%5Cu03b1%20%28Tpbpa%29%20and%20adenosine%20deaminase%20%28Ada%29%2C%20we%20identified%20a%20chimeric%20Tpbpa%5C%2FAda%20enhancer%20that%20when%20combined%20with%20the%20basal%20Ada%20promoter%20provided%20the%20highest%20luciferase%20activity%20in%20cultured%20human%20trophoblast%20cells%2C%20in%20comparison%20with%20non-trophoblast%20cell%20lines.%20We%20used%20this%20chimeric%20enhancer%20arrangement%20to%20drive%20the%20expression%20of%20a%20Cre%20recombinase%20transgene%20in%20the%20placentas%20of%20transgenic%20mice.%20Cre%20transgene%20expression%20occurred%20throughout%20the%20placenta%20but%20not%20in%20maternal%20organs%20examined%20or%20in%20the%20fetus.%5CnSIGNIFICANCE%3A%20In%20conclusion%2C%20we%20have%20provided%20both%20in%20vitro%20and%20in%20vivo%20evidence%20for%20a%20novel%20genetic%20system%20to%20achieve%20placental%20transgene%20expression%20by%20the%20use%20of%20a%20chimeric%20Tpbpa%5C%2FAda%20enhancer%20driven%20transgene.%20The%20availability%20of%20this%20expression%20vector%20provides%20transgenic%20opportunities%20to%20direct%20the%20production%20of%20desired%20proteins%20to%20the%20placenta.%22%2C%22date%22%3A%222012%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pone.0029236%22%2C%22ISSN%22%3A%221932-6203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22JH9V95Q8%22%5D%2C%22dateModified%22%3A%222018-05-22T15%3A28%3A31Z%22%7D%7D%2C%7B%22key%22%3A%22QWULCXLF%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Wu%20et%20al.%22%2C%22parsedDate%22%3A%222008-08%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWu%2C%20G.%2C%20Ai%2C%20T.%2C%20Kim%2C%20J.%20J.%20et%20al.%20%282008%29.%20%26%23x3B1%3B-1-syntrophin%20mutation%20and%20the%20long-QT%20syndrome%3A%20a%20disease%20of%20sodium%20channel%20disruption.%20%3Ci%3ECirc%20Arrhythm%20Electrophysiol%3C%5C%2Fi%3E%20%3Ci%3E1%3C%5C%2Fi%3E%2C%20193%26%23x2013%3B201.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2FCIRCEP.108.769224%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2FCIRCEP.108.769224%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22%5Cu03b1-1-syntrophin%20mutation%20and%20the%20long-QT%20syndrome%3A%20a%20disease%20of%20sodium%20channel%20disruption%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Geru%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tomohiko%22%2C%22lastName%22%3A%22Ai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeffrey%20J.%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bhagyalaxmi%22%2C%22lastName%22%3A%22Mohapatra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yutao%22%2C%22lastName%22%3A%22Xi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhaohui%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Enkhsaikhan%22%2C%22lastName%22%3A%22Purevjav%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kaveh%22%2C%22lastName%22%3A%22Samani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20J.%22%2C%22lastName%22%3A%22Ackerman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ming%22%2C%22lastName%22%3A%22Qi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arthur%20J.%22%2C%22lastName%22%3A%22Moss%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wataru%22%2C%22lastName%22%3A%22Shimizu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeffrey%20A.%22%2C%22lastName%22%3A%22Towbin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jie%22%2C%22lastName%22%3A%22Cheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matteo%22%2C%22lastName%22%3A%22Vatta%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Long-QT%20syndrome%20%28LQTS%29%20is%20an%20inherited%20disorder%20associated%20with%20sudden%20cardiac%20death.%20The%20cytoskeletal%20protein%20syntrophin-alpha%281%29%20%28SNTA1%29%20is%20known%20to%20interact%20with%20the%20cardiac%20sodium%20channel%20%28hNa%28v%291.5%29%2C%20and%20we%20hypothesized%20that%20SNTA1%20mutations%20might%20cause%20phenotypic%20LQTS%20in%20patients%20with%20genotypically%20normal%20hNa%28v%291.5%20by%20secondarily%20disturbing%20sodium%20channel%20function.%5CnMETHODS%20AND%20RESULTS%3A%20Mutational%20analysis%20of%20SNTA1%20was%20performed%20on%2039%20LQTS%20patients%20%28QTc%3E%20or%20%3D480%20ms%29%20with%20previously%20negative%20genetic%20screening%20for%20the%20known%20LQTS-causing%20genes.%20We%20identified%20a%20novel%20A257G-SNTA1%20missense%20mutation%2C%20which%20affects%20a%20highly%20conserved%20residue%2C%20in%203%20unrelated%20LQTS%20probands%20but%20not%20in%20400%20ethnic-matched%20control%20alleles.%20Only%201%20of%20these%20probands%20had%20a%20preexisting%20family%20history%20of%20LQTS%20and%20sudden%20death%20with%20an%20additional%20intronic%20variant%20in%20KCNQ1.%20Electrophysiological%20analysis%20was%20performed%20using%20HEK-293%20cells%20stably%20expressing%20hNa%28v%291.5%20and%20transiently%20transfected%20with%20either%20wild-type%20or%20mutant%20SNTA1%20and%2C%20in%20neonatal%20rat%20cardiomyocytes%2C%20transiently%20transfected%20with%20either%20wild-type%20or%20mutant%20SNTA1.%20In%20both%20HEK-293%20cells%20and%20neonatal%20rat%20cardiomyocytes%2C%20increased%20peak%20sodium%20currents%20were%20noted%20along%20with%20a%2010-mV%20negative%20shift%20of%20the%20onset%20and%20peak%20of%20currents%20of%20the%20current-voltage%20relationships.%20In%20addition%2C%20A257G-SNTA1%20shifted%20the%20steady-state%20activation%20%28V%28h%29%29%20leftward%20by%209.4%20mV%2C%20whereas%20the%20voltage-dependent%20inactivation%20kinetics%20and%20the%20late%20sodium%20currents%20were%20similar%20to%20wild-type%20SNTA1.%5CnCONCLUSION%3A%20SNTA1%20is%20a%20new%20susceptibility%20gene%20for%20LQTS.%20A257G-SNTA1%20can%20cause%20gain-of-function%20of%20Na%28v%291.5%20similar%20to%20the%20LQT3.%22%2C%22date%22%3A%22Aug%202008%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1161%5C%2FCIRCEP.108.769224%22%2C%22ISSN%22%3A%221941-3084%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%228EPJLFD3%22%2C%22JH9V95Q8%22%2C%224VC6SC4L%22%5D%2C%22dateModified%22%3A%222021-06-02T16%3A30%3A07Z%22%7D%7D%2C%7B%22key%22%3A%227SNKMM78%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mi%20et%20al.%22%2C%22parsedDate%22%3A%222008-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMi%2C%20T.%2C%20%3Cstrong%3EAbbasi%3C%5C%2Fstrong%3E%2C%20S.%2C%20Zhang%2C%20H.%20et%20al.%20%282008%29.%20Excess%20adenosine%20in%20murine%20penile%20erectile%20tissues%20contributes%20to%20priapism%20via%20A2B%20adenosine%20receptor%20signaling.%20%3Ci%3EJ%20Clin%20Invest%3C%5C%2Fi%3E%20%3Ci%3E118%3C%5C%2Fi%3E%2C%201491%26%23x2013%3B1501.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1172%5C%2FJCI33467%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1172%5C%2FJCI33467%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Excess%20adenosine%20in%20murine%20penile%20erectile%20tissues%20contributes%20to%20priapism%20via%20A2B%20adenosine%20receptor%20signaling%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tiejuan%22%2C%22lastName%22%3A%22Mi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hong%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karen%22%2C%22lastName%22%3A%22Uray%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Janci%20L.%22%2C%22lastName%22%3A%22Chunn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ling%20Wei%22%2C%22lastName%22%3A%22Xia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jose%20G.%22%2C%22lastName%22%3A%22Molina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Norman%20W.%22%2C%22lastName%22%3A%22Weisbrodt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rodney%20E.%22%2C%22lastName%22%3A%22Kellems%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20R.%22%2C%22lastName%22%3A%22Blackburn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yang%22%2C%22lastName%22%3A%22Xia%22%7D%5D%2C%22abstractNote%22%3A%22Priapism%2C%20abnormally%20prolonged%20penile%20erection%20in%20the%20absence%20of%20sexual%20excitation%2C%20is%20associated%20with%20ischemia-mediated%20erectile%20tissue%20damage%20and%20subsequent%20erectile%20dysfunction.%20It%20is%20common%20among%20males%20with%20sickle%20cell%20disease%20%28SCD%29%2C%20and%20SCD%20transgenic%20mice%20are%20an%20accepted%20model%20of%20the%20disorder.%20Current%20strategies%20to%20manage%20priapism%20suffer%20from%20a%20poor%20fundamental%20understanding%20of%20the%20molecular%20mechanisms%20underlying%20the%20disorder.%20Here%20we%20report%20that%20mice%20lacking%20adenosine%20deaminase%20%28ADA%29%2C%20an%20enzyme%20necessary%20for%20the%20breakdown%20of%20adenosine%2C%20displayed%20unexpected%20priapic%20activity.%20ADA%20enzyme%20therapy%20successfully%20corrected%20the%20priapic%20activity%20both%20in%20vivo%20and%20in%20vitro%2C%20suggesting%20that%20it%20was%20dependent%20on%20elevated%20adenosine%20levels.%20Further%20genetic%20and%20pharmacologic%20evidence%20demonstrated%20that%20A2B%20adenosine%20receptor-mediated%20%28A2BR-mediated%29%20cAMP%20and%20cGMP%20induction%20was%20required%20for%20elevated%20adenosine-induced%20prolonged%20penile%20erection.%20Finally%2C%20priapic%20activity%20in%20SCD%20transgenic%20mice%20was%20also%20caused%20by%20elevated%20adenosine%20levels%20and%20A2BR%20activation.%20Thus%2C%20we%20have%20shown%20that%20excessive%20adenosine%20accumulation%20in%20the%20penis%20contributes%20to%20priapism%20through%20increased%20A2BR%20signaling%20in%20both%20Ada%20-%5C%2F-%20and%20SCD%20transgenic%20mice.%20These%20findings%20provide%20insight%20regarding%20the%20molecular%20basis%20of%20priapism%20and%20suggest%20that%20strategies%20to%20either%20reduce%20adenosine%20or%20block%20A2BR%20activation%20may%20prove%20beneficial%20in%20the%20treatment%20of%20this%20disorder.%22%2C%22date%22%3A%22Apr%202008%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1172%5C%2FJCI33467%22%2C%22ISSN%22%3A%220021-9738%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22JH9V95Q8%22%5D%2C%22dateModified%22%3A%222018-05-22T15%3A28%3A31Z%22%7D%7D%2C%7B%22key%22%3A%22JFYV5ZKM%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zhou%20et%20al.%22%2C%22parsedDate%22%3A%222007-01-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZhou%2C%20C.%20C.%2C%20Ahmad%2C%20S.%2C%20Mi%2C%20T.%20et%20al.%20%282007%29.%20Angiotensin%20II%20induces%20soluble%20fms-Like%20tyrosine%20kinase-1%20release%20via%20calcineurin%20signaling%20pathway%20in%20pregnancy.%20%3Ci%3ECirc%20Res%3C%5C%2Fi%3E%20%3Ci%3E100%3C%5C%2Fi%3E%2C%2088%26%23x2013%3B95.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2F01.RES.0000254703.11154.18%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2F01.RES.0000254703.11154.18%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Angiotensin%20II%20induces%20soluble%20fms-Like%20tyrosine%20kinase-1%20release%20via%20calcineurin%20signaling%20pathway%20in%20pregnancy%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cissy%20Chenyi%22%2C%22lastName%22%3A%22Zhou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shakil%22%2C%22lastName%22%3A%22Ahmad%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22TieJuan%22%2C%22lastName%22%3A%22Mi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lingwei%22%2C%22lastName%22%3A%22Xia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shahrzad%22%2C%22lastName%22%3A%22Abbasi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%20W.%22%2C%22lastName%22%3A%22Hewett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22ChunXiao%22%2C%22lastName%22%3A%22Sun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Asif%22%2C%22lastName%22%3A%22Ahmed%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rodney%20E.%22%2C%22lastName%22%3A%22Kellems%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yang%22%2C%22lastName%22%3A%22Xia%22%7D%5D%2C%22abstractNote%22%3A%22Maternal%20endothelial%20dysfunction%20in%20preeclampsia%20is%20associated%20with%20increased%20soluble%20fms-like%20tyrosine%20kinase-1%20%28sFlt-1%29%2C%20a%20circulating%20antagonist%20of%20vascular%20endothelial%20growth%20factor%20and%20placental%20growth%20factor.%20Angiotensin%20II%20%28Ang%20II%29%20is%20a%20potent%20vasoconstrictor%20that%20increases%20concomitant%20with%20sFlt-1%20during%20pregnancy.%20Therefore%2C%20we%20speculated%20that%20Ang%20II%20may%20promote%20the%20expression%20of%20sFlt-1%20in%20pregnancy.%20Here%20we%20report%20that%20infusion%20of%20Ang%20II%20significantly%20increases%20circulating%20levels%20of%20sFlt-1%20in%20pregnant%20mice%2C%20thereby%20demonstrating%20that%20Ang%20II%20is%20a%20regulator%20of%20sFlt-1%20secretion%20in%20vivo.%20Furthermore%2C%20Ang%20II%20stimulated%20sFlt-1%20production%20in%20a%20dose-%20and%20time-dependent%20manner%20from%20human%20villous%20explants%20and%20cultured%20trophoblasts%20but%20not%20from%20endothelial%20cells%2C%20suggesting%20that%20trophoblasts%20are%20the%20primary%20source%20of%20sFlt-1%20during%20pregnancy.%20As%20expected%2C%20Ang%20II-induced%20sFlt-1%20secretion%20resulted%20in%20the%20inhibition%20of%20endothelial%20cell%20migration%20and%20in%20vitro%20tube%20formation.%20In%20vitro%20and%20in%20vivo%20studies%20with%20losartan%2C%20small%20interfering%20RNA%20specific%20for%20calcineurin%20and%20FK506%20demonstrated%20that%20Ang%20II-mediated%20sFlt-1%20release%20was%20via%20Ang%20II%20type%201%20receptor%20activation%20and%20calcineurin%20signaling%2C%20respectively.%20These%20findings%20reveal%20a%20previously%20unrecognized%20regulatory%20role%20for%20Ang%20II%20on%20sFlt-1%20expression%20in%20murine%20and%20human%20pregnancy%20and%20suggest%20that%20elevated%20sFlt-1%20levels%20in%20preeclampsia%20may%20be%20caused%20by%20a%20dysregulation%20of%20the%20local%20renin%5C%2Fangiotensin%20system.%22%2C%22date%22%3A%22Jan%2005%2C%202007%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1161%5C%2F01.RES.0000254703.11154.18%22%2C%22ISSN%22%3A%221524-4571%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22JH9V95Q8%22%5D%2C%22dateModified%22%3A%222018-05-22T15%3A28%3A31Z%22%7D%7D%5D%7D
Ma, Y., Cheng, N., Sun, J. et al. (2020). Atherogenic L5 LDL induces cardiomyocyte apoptosis and inhibits KATP channels through CaMKII activation. Lipids Health Dis 19, 189. https://doi.org/10.1186/s12944-020-01368-7.
Woodside, D. G., Tanifum, E. A., Ghaghada, K. B. et al. (2018). Magnetic resonance imaging of atherosclerotic plaque at clinically relevant field strengths (1T) by targeting the integrin α4β1. Sci Rep 8, 3733. https://doi.org/10.1038/s41598-018-21893-x.
Xi, Y., James Chao, Z.-Y., Yan, W. et al. (2015). Neuronally released vasoactive intestinal polypeptide alters atrial electrophysiological properties and may promote atrial fibrillation. Heart Rhythm 12, 1352–1361. https://doi.org/10.1016/j.hrthm.2015.03.003.
Sun, J., Zhang, J., Yan, W. et al. (2014). Iloprost prevents doxorubicin mediated human cardiac progenitor cell depletion. Int J Cardiol 176, 536–539. https://doi.org/10.1016/j.ijcard.2014.07.031.
Xi, Y., Wu, G., Ai, T. et al. (2013). Ionic mechanisms underlying the effects of vasoactive intestinal polypeptide on canine atrial myocardium. Circ Arrhythm Electrophysiol 6, 976–983. https://doi.org/10.1161/CIRCEP.113.000518.
Barakat, O., Abbasi, S., Rodriguez, G. et al. (2012). Use of decellularized porcine liver for engineering humanized liver organ. J Surg Res 173, e11-25. https://doi.org/10.1016/j.jss.2011.09.033.
Zhou, C. C., Chang, J., Mi, T. et al. (2012). Targeted expression of Cre recombinase provokes placental-specific DNA recombination in transgenic mice. PLoS ONE 7, e29236. https://doi.org/10.1371/journal.pone.0029236.
Wu, G., Ai, T., Kim, J. J. et al. (2008). α-1-syntrophin mutation and the long-QT syndrome: a disease of sodium channel disruption. Circ Arrhythm Electrophysiol 1, 193–201. https://doi.org/10.1161/CIRCEP.108.769224.
Mi, T., Abbasi, S., Zhang, H. et al. (2008). Excess adenosine in murine penile erectile tissues contributes to priapism via A2B adenosine receptor signaling. J Clin Invest 118, 1491–1501. https://doi.org/10.1172/JCI33467.
Zhou, C. C., Ahmad, S., Mi, T. et al. (2007). Angiotensin II induces soluble fms-Like tyrosine kinase-1 release via calcineurin signaling pathway in pregnancy. Circ Res 100, 88–95. https://doi.org/10.1161/01.RES.0000254703.11154.18.